Journal of Radiation Research and Radiation Proces ›› 2018, Vol. 36 ›› Issue (3): 30201-030201.doi: 10.11889/j.1000-3436.2018.rrj.36.030201

• RADIOBIOLOGY AND RADIOMEDICINE •     Next Articles

Protective effect of glycyrrhizin magnesium (Gly-Mg) on radiation injury in mice

MU Gan'en, JIA Yiyue, LI Yuanyuan, SHEN Xiu, ZHOU Zewei   

  1. Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China
  • Received:2018-02-05 Revised:2018-03-18 Online:2018-06-20 Published:2018-06-21
  • Supported by:

    Supported by Tianjin Application Foundation and Research in Cutting-edge Technologies Program (15JCZDJC33400)

Abstract:

This study utilized antioxidant and basic single cell gel electrophoresis experiments to assess the effect of glycyrrhizin magnesium (Gly-Mg) on oxidative and DNA damage. ICR (Institute of Cancer Research) mice were divided into a control group (normal saline), irradiation control group (antioxidant experiment:normal saline+6.0 Gy; comet test:normal saline+7.2 Gy), and Gly-Mg group (antioxidant experiment:50 mg/kg+6.0 Gy, 75 mg/kg+6.0 Gy; comet test:50 mg/kg+7.2 Gy, 75 mg/kg+7.2 Gy). The activity of superoxide dismutase (SOD) and catalase (CAT), and the content of glutathione (GSH) and methylene dioxyamphetamine (MDA) in the serum, liver, and lung tissues of mice were detected using antioxidant kits. Results of the oxidative damage protection tests suggested that Gly-Mg can improve the activities of SOD and CAT, increase the content of GSH, and reduce the content of MDA in mouse serum and tissues. Meanwhile, results of the comet test showed that Gly-Mg groups had a significant protective effect on radiation-induced DNA damage in bone marrow compared with that in the irradiation control group (p<0.01). In conclusion, Gly-Mg has protective effect against antioxidant and DNA damage.

Key words: Glycyrrhizin magnesium (Gly-Mg), Radioprotection, Antioxidation, Comet assay

CLC Number: 

  • R81