Journal of Radiation Research and Radiation Proces ›› 2018, Vol. 36 ›› Issue (1): 10202-010202.doi: 10.11889/j.1000-3436.2018.rrj.36.010202

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Cumulative effect of wide-band electromagnetic pulse exposure on the reproductive system of male rats

LEI Mingjia, LIU He, ZHANG Jie, QI Hongxin, WANG Xianghui   

  1. Laboratory of Biophysics, Department of Physics and Materials Science, East China Normal University, Shanghai 200241, China
  • Received:2017-09-30 Revised:2017-11-30 Online:2018-02-20 Published:2018-02-08
  • Supported by:

    Supported by National Natural Science Foundation of China (31600675)

Abstract:

The present work focused on the cumulative damage caused by periodically repeated wide-band electromagnetic pulses. The exposure protocol was as follows: electric field intensity E=110 kV/m, 297 kV/m, and 873 kV/m; pulse number N=5, and 100; exposure was repeated 2 times per day, for 2 weeks. 6 h, 1 d, 3 d, 7 d, and 10 d after exposure, the testosterone concentration in blood plasma, superoxide dismutase (SOD) and malondialdehyde (MDA) in the testis, and acrosin activity of sperms were determined by ELISA. The apoptosis rate of spermatogenic cells in the testis was studied by TUNEL, and the survival rate of sperms in the epididymis by CASA. The results showed that compared with single exposure, 28 periodic exposures exhibited a cumulative damaging effect on the reproductive system of male rats. The cumulative effect increased significantly with increasing E and N. Similar to single exposure, the damage caused by repetitive exposure was gradually recovered to a certain degree with prolongation of exposure time.

Key words: Wide-band electromagnetic pulse, Repeated exposure, Cumulative damage, Reproductive system, Sperm

CLC Number: 

  • TL71