Journal of Radiation Research and Radiation Proces ›› 2018, Vol. 36 ›› Issue (1): 10201-010201.doi: 10.11889/j.1000-3436.2018.rrj.36.010201

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Protective effect of edaravone on hematopoietic system injury induced by ionizing radiation in mice

ZHANG Qianru, ZHANG Junling, TIAN Hongqi   

  1. Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China
  • Received:2017-10-18 Revised:2017-12-15 Online:2018-02-20 Published:2018-02-08
  • Supported by:

    Supported by Fundamental Research Fund for CAMS&PUMC (2016ZX310199), CAMS Innovation Fund for Medical Sciences (CIFMS, 2017-I2M-3-019), National Natural Science Foundation of China (81402633), and the IRM-CAMS Research and Innovation Team (1649)

Abstract:

Twenty C57BL/6 mice were randomly divided into four groups equally: control, edaravone, radiation (4 Gy), and radiation with edaravone (4 Gy+Eda). Edaravone was dissolved in normal saline at concentration of 45 mg/mL. Mice in the edaravone, and 4 Gy+Eda groups were injected with edaravone intraperitoneally, while mice in control, and 4 Gy groups were injected with normal saline intraperitoneally. Mice in 4 Gy, and 4 Gy+Eda groups were then irradiated by 137Cs γ-rays at a dose of 4 Gy under dose rate of 0.99 Gy/min 30 min after injection. Mice in these four groups were euthanized 15 d after irradiation. Hematology analyzer was used to measure cell counts in peripheral blood. Flow cytometry was used to analyze the percentage of hematopoietic stem and progenitor cells (HSPCs) in bone marrow cells, as well as the levels of reactive oxygen species (ROS) in HSPCs. Colony formation unit-granulocyte macrophage (CFU-GM) assay was used to detect the proliferation of HSPCs. The results showed that compared with those of the control group, the number of peripheral blood leucocytes (WBC), erythrocytes (RBC), and platelet (PLT) in mice in 4 Gy group decreased by 74.16%, 16.63%, and 15.04%, respectively; the percentage of HPC, LSK(Lin- Sca-1+ c-kit+), and CD34+LSK cells decreased by 50.99%, 38.51%, and 14.84%, respectively; the number of CFU-GM in bone marrow cells decreased by 93.44%, and the proportion of CD34-LSK cells increased by 1.74 times; the levels of ROS in HPC and LSK cells increased by 44.11% and 61.43%, respectively. Compared with those of the 4 Gy group, the number of WBC and RBC in mice in 4 Gy+Eda group increased by 58.82% and 9.3%, respectively; the proportion of HPC, LSK, and CD34+LSK cells increased by 1.53 times, 1.5 times, and 14.81%, respectively; the number of CFU-GM in bone marrow cells increased by 57.58%, the percentage of CD34-LSK cells decreased by 49.17%, and the ROS levels of HPC and LSK cells decreased by 38.94% and 55.91%, respectively (p<0.05). Edaravone exhibits a protective effect on radiation-induced hematopoietic system injury.

Key words: Edaravone, Radioprotector, Mice, Hematopoietic system, Reactive oxygen species (ROS)

CLC Number: 

  • TL71