Journal of Radiation Research and Radiation Proces ›› 2017, Vol. 35 ›› Issue (6): 60401-060401.doi: 10.11889/j.1000-3436.2017.rrj.35.060401

• RADIATION TECHNOLOGY APPLICATION • Previous Articles     Next Articles

Screening and mutation breeding of high-yield Cordyceps militaris via heavy ion irradiation

JIA Guojun1, MA Zhengui1, ZHAO Fengwu1,2, LU Xihong2, WANG Guirong3   

  1. 1 Department of Bioengineering, Lanzhou Vocational Technical College, Lanzhou 730070, China;
    2 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
    3 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2017-08-18 Revised:2017-09-29 Online:2017-12-20 Published:2017-12-20
  • Supported by:

    Supported by Lanzhou talent innovation and entrepreneurship project (2015-RC-21), Lanzhou science and technology plan project (2016-3-2), and Lanzhou Vocational Technical College project (2016B-1)

Abstract:

Strains of Cordyceps militaris grown in suspension culture were irradiated by 12C6+ ion beam, screened using the plate separation method, and re-screened by culturing fruiting bodies to determine the fruiting-body yield, polysaccharide and cordycepin content. The strains displayed the highest lethality, i.e., 82%, at the optimal absorbed dose of 150 Gy. Three mutants (G2, G5, G12) were obtained, which could produce a greater number of fruiting bodies. Meanwhile, the ten times pass-generation test indicated that the fruiting bodies stabilized at 8.02 to 8.21 g/bottle in these three mutants, which were 52.47% to 56.08% higher than the original strain, and the hereditary characters of these mutants were stable. The present findings show that a high-yield fruiting body of C.militaris can be obtained by mutation breeding using a heavy ion beam irradiation.

Key words: Cordyceps militaris, Heavy ion beams, Mutation breeding, Irradiation

CLC Number: 

  • TL99