Journal of Radiation Research and Radiation Proces ›› 2017, Vol. 35 ›› Issue (2): 20202-020202.doi: 10.11889/j.1000-3436.2017.rrj.35.020202

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Bioinformatic analysis of microRNA expression profiles in X-ray-irradiated CAL-27 cells

HU Songling1,2, WANG Weiping1, LU Yuying1, YANG Luo1, WU Qingfeng2, DANG Bingrong2, HE Xiangyi1   

  1. 1. School of Stomatology, Lanzhou University, Lanzhou 730000, China;
    2. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
  • Received:2017-01-16 Revised:2017-03-17 Online:2017-04-20 Published:2017-04-14
  • Supported by:

    Supported by the National Natural Science Foundation Joint Funds (Y206530GJ0)


CAL-27, a human tongue squamous carcinoma cell line was selected for the experiment. The cell clone formation rate was detected by a cell clone forming experiment and flow cytometry was used to detect apoptosis. High throughput sequencing technique (HiSeq) was applied for detecting the differentially expressed miRNAs (DEGs) after irradiating CAL-27 cells with X-rays. Using bioinformatics analysis, the distribution of candidate target genes and significantly enriched GO-term genes were obtained. The main signaling pathways involving the DEGs were obtained using KEGG pathway enrichment analysis. The CAL-27 cell clone survival rate of the 2 Gy irradiated group was 59% and the apoptosis rate was 47.5% (p<0.05). After irradiation, 21 miRNAs were altered, of which 18 miRNAs were upregulated (hsa-miR-21-3p, hsa-miR-101-3p, hsa-miR-100-3p, etc.) and 3 miRNAs were downregulated (hsa-miR-204-5p, hsa-miR-10a-5p, hsa-miR-146b-5p). When analyzed by GO function, there were 23 GO-terms enriched in biological process, 20 GO-terms enriched in molecular function, and 18 GO-terms enriched in cellular component. Furthermore, 18 KEGG pathways in which the DEGs participated, were screened out including olfactory transduction, cytokine-cytokine receptor interaction, MAPK signaling pathway-yeast (p<0.05), etc. These results indicate that X-ray irradiation with a dose of 2 Gy altered the miRNA expression profile of CAL-27 cells, and the underlying mechanism may involve MAPK signaling pathways. This study lays a foundation for further study of miRNAs in the molecular mechanism of tongue squamous carcinoma radiotherapy.

Key words: X-ray irradiation, CAL-27 cell, MicroRNA

CLC Number: 

  • Q691.5