Journal of Radiation Research and Radiation Proces ›› 2017, Vol. 35 ›› Issue (2): 20201-020201.doi: 10.11889/j.1000-3436.2017.rrj.35.020201

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Protective effect of resveratrol on UVA-irradiated HaCaT cells and its mechanism

LI Chen, WANG Chunyan, TIAN Mei, QI Xuesong, SHAO Shuai, GOU Qiao   

  1. National Institute for Radiological Protection, Key Laboratory of Radiological Protection and Nuclear Emergency, Chinese Center for Disease Control and Prevention, Beijing 100088, China
  • Received:2017-01-04 Revised:2017-02-22 Online:2017-04-20 Published:2017-04-14

Abstract:

The Cell Counting Kit-8 (CCK-8) was used to detect the cell survival rate of HaCaT cells. The apoptosis rate and mortality of HaCaT cells were detected using flow cytometry with the Annexin VFITC and PI double labeling method. Autophagosomes and the generation of reactive oxygen species (ROS) in HaCaT cells were monitored by laser scanning confocal microscopy using monodansylcadaverine (MDC) and dichlorodihydrofluorescein diacetate (DCFH-DA) staining, respectively. The expression of LC3B-II, p-AKT, and total AKT protein in HaCaT cells was determined by western blotting. The results showed that HaCaT cell proliferation was inhibited gradually (p<0.05) after irradiation with a dose of 10~80 J/cm2 UVA. Treatment with resveratrol enhanced the proliferation of UVA-irradiated HaCaT cells (p<0.01) and decreased their apoptosis rate and mortality (p<0.05). The expression of LC3B-II protein was increased (p<0.01) after irradiation with 20 J/cm2 UVA for 3~24 h. After treatment with resveratrol, autophagy was enhanced (p<0.01), intracellular ROS level was decreased (p<0.01), and p-AKT expression was significantly increased (p<0.01) in UVA-irradiated HaCaT cells. These results indicate that resveratrol could reduce UVA-induced proliferative inhibition, apoptosis, and death in HaCaT cells by enhancing the autophagic process and p-AKT protein expression and by reducing ROS production.

Key words: Resveratrol, HaCaT cells, UVA, Protective effect

CLC Number: 

  • TL75