Journal of Radiation Research and Radiation Proces ›› 2017, Vol. 35 ›› Issue (1): 10202-010202.doi: 10.11889/j.1000-3436.2017.rrj.35.010202

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Effects of radiation-frequency electromagnetic radiation on cholinergic neuronal markers of SD rat brain

ZHOU Jingcheng1, LIN Yanyun2, CHEN Keliang1, GAO Peng2, WU Tao2, LIU Junye2, ZENG Lihua2, GUO Guozhen2   

  1. 1 The Fourth Military Medical University, Xi'an 710032, China;
    2 Department of Radiation Medicine, the Fourth Military Medical University, Xi'an 710032, China
  • Received:2016-08-25 Revised:2016-11-27 Online:2017-02-20 Published:2017-02-20
  • Supported by:

    Supported by National Basic Research Program of China (973 Program)(2011CB503704),National Natural Science Foundation of China (51677189,51437008),and Natural Science Foundation of Shanxi Province (2015JM8454)

Abstract:

Twenty-four male SD rats were randomly assigned to 4 groups:sham-exposure group, 0.2 W/kg exposure group, 0.5 W/kg exposure group, and 1.5 W/kg exposure group. The exposure groups were exposed to 1.84 GHz, with SAR (Specific absorption rate) of 0.2, 0.5, 1.5 W/kg for 2 weeks of 5 d/week and 30 min/d. Immediately after the last exposure, SD rats were anesthetized and executed, and then cerebral cortex and hippocampus were collected. Chemical colorimetry assay was used to detect the levels of cholinergic neuronal markers, the activity of antioxidant enzymes and the products induced by oxidative stress in cerebral cortex and hippocampus. The results showed that compared with that in the sham-exposure group, the ACh and ChAT level of rat cerebral cortex decreased significantly in 1.5 W/kg exposure group (p<0.05); the AChE level of rat cerebral cortex didn't show any obvious difference in exposure groups; the activity of SOD, GPX, CAT and MDA contents didn't change significantly in exposure groups; the level of cholinergic neuronal markers, the activity of antioxidant enzymes and the products induced by oxidative stress in hippocampus didn't have any significant difference in the exposure groups. The results indicate that under the present experimental setting, radiation does not cause brain damage induced by oxidative stress, and some cholinergic neuronal markers level in the cerebral cortex of SD rat decrease in 1.5 W/kg exposure group.

Key words: Radiation-frequency electromagnetic radiation, SD rat, Cholinergic neuronal markers, Antioxidative damage, Brain tissue

CLC Number: 

  • TL71