Journal of Radiation Research and Radiation Proces ›› 2017, Vol. 35 ›› Issue (1): 10201-010201.doi: 10.11889/j.1000-3436.2017.rrj.35.010201

• RADIOBIOLOGY AND RADIOMEDICINE •     Next Articles

Syntheses and radiosensitization effect of mitochondria-targeted 6-(4-(dimethylamine) pyridine) methyl coumarin

WANG Huanan, YAO Ming, XU Wenqing   

  1. Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College & Chinese Academy of Medical Science, Tianjin 300192, China
  • Received:2016-09-02 Revised:2016-09-29 Online:2017-02-20 Published:2017-02-20
  • Supported by:

    Supported by Tianjin Research Program of Application Foundation and Advanced Technology (14JCZDJC36400),and Chinese Academy of Medical Sciences Youth Exploration Fund (2016ZX310073)

Abstract:

6-(4-(dimethylamine) pyridine) methyl coumarin with antitumor and radio sensitizing effect on A549 cells was synthesized through brominating and electrophonic reactions. The inhibition rate for A549 cells, the radio sensitizing effect, and the reactive oxygen species (ROS) volume were measured by MTT assay, clone formation assay (radio sensitizing effect (RSE) was calculated by the multitarget single hit model) and DCFH-DA method, respectively. The results showed that the inhibition rate for A549 cells was positive related to the drug concentration, and the survival fraction of combination group (radiation plus administration drug group) decreased significantly compared with radiation group (p<0.05), RSE=1.585, the ROS volume of combination group increased significantly compared with radiation group (p<0.05) under various doses. The results indicate that 6-(4-(dimethylamine) pyridine) methyl coumarin can inhibit A549 cells and the inhibition effect increase with the drug dose, and has radio sensitizing effect on A549 cells, and can increase the ROS volume in A549 cells significantly.

Key words: 6-(4-(dimethylamine) pyridine) methyl coumarin, Radiosensitization, A549 cells

CLC Number: 

  • Q691