Journal of Radiation Research and Radiation Proces ›› 2016, Vol. 34 ›› Issue (6): 60201-60201.doi: 10.11889/j.1000-3436.2016.rrj.34.060201

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Effects of Anduolin on Caspase-9 and XIAP of testicular cells in rats irradiated by high-power microwave

HAO Shuxia, LYU Huimin, WANG Chunyan, QI Xuesong, TONG Peng, GOU Qiao   

  1. National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China
  • Received:2016-07-06 Revised:2016-09-19 Online:2016-12-20 Published:2016-12-08

Abstract:

In order to investigate the Anduolin injury prevention mechanism of microwave exposure, sexually matured SD rats were randomly divided into control group, exposure group, and prevention groups (drug dosage were 3, 6 and 9 mg/kg). Prevention groups were given drug continuously by oral administration for 20 d, meanwhile the control group and exposure group were given distilled water. After that the prevention groups and exposure group were exposed to high power-microwave (HPM) of 100 mW/cm2 for 10 min. Rats were killed at 1st, 2nd, and 5th day after exposure, and the pathological specimens were prepared from testicular tissue. Interested proteins in testicular tissue were detected with immunohistochemical method, and the protein content were analyzed with image-pro plus analysis software. The results showed that protein content of Caspase-9 of exposure group showed a high-low-high trend compared with control group (p<0.05) at 1st, 2nd, and 5th day after exposed to HPM, while the prevention groups showed a trend from low to high with time (p<0.05); for X-linked inhibitor of apoptosis protein (XIAP) protein, there was no significantly difference between exposure group and control group, while the XIAP levels of 6 mg/kg group and 9 mg/kg group at 5th day after exposure increased significantly compared with the exposure group (p<0.05). The results suggest that Caspase-9 is involved in the HPM-induced apoptosis in rat testis, and protective effect of Anduolin on rat testicular cells requires participation of Caspase-9 and XIAP.

Key words: High power microwave, Anduolin, Apoptosis, Caspase-9, X-linked inhibitor of apoptosis protein (XIAP)

CLC Number: 

  • TL7