Journal of Radiation Research and Radiation Proces ›› 2016, Vol. 34 ›› Issue (5): 50203-050203.doi: 10.11889/j.1000-3436.2016.rrj.34.050203

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Cloning, and functional analysis of DR_2327 and DR_2328 in Deinococcus radiodurans

YANG Yuhong   

  1. Yongzhou Vocational Technical College, Yongzhou 425100, China
  • Received:2016-06-06 Revised:2016-07-07 Online:2016-10-20 Published:2016-10-14

Abstract:

The main characteristics of DR_2327and DR_2328 in Deinococcus radiodurans were analyzeed by bioinformatics. Full-length gene of DR_2327 and DR_2328 was amplified by PCR, and constructed in pGEM-T vector. Transformed recombinant vector into E.coli JM109 and followed by restriction enzyme digestion. DR_2327 and DR_2328 gene was cloned successfully. Bioinformatic analysis results showed that DR_2327 and DR_2328 gene located in the same operon, and DR_2328 protein with histidine kinase activity, DR_2327 protein with response regulator activity. It is suggested that DR_2327 and DR_2328 constitute a two-component system in Deinococcus radiodurans. In vitro experiments showed that DR_2327 and DR_2328 could enhance tolerance to UV radiation and H2O2 of E.coli.

Key words: Deinococcus radiodurans, DR_2327, DR_2328, Gene cloning, Bioinformatics analysis

CLC Number: 

  • Q78