Journal of Radiation Research and Radiation Proces ›› 2016, Vol. 34 ›› Issue (5): 50202-050202.doi: 10.11889/j.1000-3436.2016.rrj.34.050202

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Interference of ATM gene expression increasing the radiosensitivity of AGS cells

ZHENG Xu, LIU Dan, LIU Dawei, LIU Fan   

  1. Department of Radiation Oncology, the Fourth Affiliated Hospital of China Medical University, Shenyang 110032, China
  • Received:2016-04-27 Revised:2016-06-03 Online:2016-10-20 Published:2016-10-14

Abstract:

To investigate the interference effect of ataxia-telangiectasia mutated (ATM) gene expression on the radiosensitivity of human gastric cancer AGS cells, the plasmid of interfering RNA was used to transfect AGS cells. The mRNA and protein expression levels of ATM gene were measured by RT-PCR and Western blot, respectively. The effect on cell growth after irradiation was analyzed by using the colonies formation assay. Cell cycle and cell apoptosis were analyzed by flow cytometry. The RNA interference plasmid of ATM gene was transfected into AGS cells and AGS-Ri-ATM cells were obtained successfully. The RT-PCR and Western blot results demonstrated that the expression of ATM gene was down-regulated in the AGS-Ri-ATM cells. The colonies formation numbers/ratios of AGS-Ri-ATM cells were less than those of AGS-Vector. The flow cytometry demonstrated that G1 arrest was induced and apoptosis appeared in AGS-Ri-ATM cells after irradiation at 4 Gy for 12 h, which was not observed in AGS-Vector cells. Interference of ATM expression increased the radiosensitivity of human gastric cancer AGS cells.

Key words: Ataxia-telangiectasia mutated (ATM) gene, RNA interference, Gastric cancer, Radiosensitivity

CLC Number: 

  • Q691