1,4-二氢吡啶类化合物对CHO-K1细胞的辐射防护作用

doi:10.11889/j.1000-3436.2016.rrj.34.040601

Journal of Radiation Research and Radiation Proces ›› 2016, Vol. 34 ›› Issue (4): 40601-040601.doi: 10.11889/j.1000-3436.2016.rrj.34.040601

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Radioprotective effect of 1,4-dihydropyridine in CHO-K1 cell

ZHANG Yurui, XUE Xuhui, LI Yuanyuan, XU Wenqing

Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College & Chinese Academy of Medical Science, Tianjin 300192, China

Received:2016-03-18 Revised:2016-04-28 Online:2016-08-20 Published:2016-08-18
Supported by:
Supported by National Natural Science Foundation of China (81273005), Tianjin Municipal Science and Technology Commission (14JCZDJC36400) and the IRM-CAMS Research Fund (SF1528)

Abstract

Abstract:

The aim is to study the radiation protection effect of 1,4-dihydropyridine in CHO-K1 cell. There were three groups which were DHP treated group, radiation group and control group, respectively. Diethyl 2,6- dimethyl-1,4-dihydropyridine-3,5-dicarboxylate (DHP) cell viability and cell cytotoxicity were measured by neutral red assay, DCFH-DA was used to test the level of reactive oxygen species (ROS) and comet assay was adopted to measure DNA damages. Radioprotective activity was evaluated by these three indices. The fluorescence intensity of DCF was 1 800, tail DNA and tail moment were 3.5% and 1.7% in radiation group, which significantly increased (p<0.05) compared with control group. The results indicated that radiation could produce more ROS to cause DNA damage. The fluorescence intensity of DCF was 945, tail DNA and tail moment were 0.83% and 0.7% in DHP treated group, which significantly decreased (p<0.05) compared with radiation group. The results indicated that radiation could induce DNA damage and DHP could relief this injury to DNA. It can be concluded that DHP could protect cell against radiation-induced damage in CHO-K1 cell.

Key words: 1,4-dihydropyridine, Radioprotector, Reactive oxygen species, CHO-K1 cell

CLC Number:

ZHANG Yurui, XUE Xuhui, LI Yuanyuan, XU Wenqing. Radioprotective effect of 1,4-dihydropyridine in CHO-K1 cell[J].Journal of Radiation Research and Radiation Proces, 2016, 34(4): 40601-040601.

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Radioprotective effect of 1,4-dihydropyridine in CHO-K1 cell

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