Journal of Radiation Research and Radiation Proces ›› 2016, Vol. 34 ›› Issue (2): 20205-020205.doi: 10.11889/j.1000-3436.2016.rrj.34.020205

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Protection effects of carboxymethyl-β-1,3-glucan on human B lymphoblasts' injury induced by radiation

MA Binbo1,2, WANG Zhuanzi2, WEI Wei2, DANG Bingrong2, LI Wenjian1,2   

  1. 1. School of Pharmacy, Lanzhou University, Lanzhou 730000, China;
    2. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
  • Received:2015-10-12 Revised:2016-01-18 Online:2016-04-20 Published:2016-04-08
  • Supported by:

    Supported by the National Natural Science Foundation of China (11575259)

Abstract:

The aim is to investigate radiation protection effect induced by carboxymethyl-β-1,3-glucan on Human B lymphoblasts(HMy2.CIR). The survival rate of carboxymethyl-β-1,3-glucan on HMy2.CIR was tested by CCK-8 assay at concentrations of 0, 0.01, 0.1, 1, 100, 200 and 400 μg/mL, respectively. The flow cytometry was used to measure the apoptosis rate. Micronucleus rate was assessed by the in vitro micronucleus test. DNA strand breaks were assessed by the comet assay. The results showed that treatment with carboxymethyl-β-1,3-glucan was not cytotoxic. Pre-treatment with carboxymethyl-β-1,3-glucan at concentration of 0.1 μg/mL for 72 h protected HMy2.CIR cells against radiation, as indicated by increased surviving fraction, reduced apoptosis, reduced micronucleus rate and fewer DNA strand breaks. The carboxymethyl-β-1,3-glucan has radiation protection effect on HMy2.CIR cells, which may be related to inhibition of apoptosis and DNA damage after radiation.

Key words: Carboxymethy-β-1, 3-glucan, Human B lymphoblasts, X-ray radiation, Radiation protection effect

CLC Number: 

  • R979.6