Journal of Radiation Research and Radiation Proces ›› 2016, Vol. 34 ›› Issue (2): 20203-020203.doi: 10.11889/j.1000-3436.2016.rrj.34.020203

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Protective effects of the plant lectin PHA-L on acute radiation injuries in mice

ZHANG Guanghui, WU Hongying, JIN Jin, ZHOU Zewei, LIU Peixun, LONG Wei   

  1. Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China
  • Received:2015-10-29 Revised:2015-11-26 Online:2016-04-20 Published:2016-03-17
  • Supported by:

    Supported by National Natural Science Foundation of China(81202153), Doctoral Found of Ministry of Education(20121106120042), Tianjin application foundation and research in cutting-edge technologies program(14JCQNJC10900), and Development Fund of Institute of Radiation Medicine, Chinese Academy of Medical Sciences(1523)

Abstract:

The aim is to observe the protective effects of plant lectin PHA-L against radiation injuries and the relative mechanism. 30 d survival experiment was used to observe the survival condition of mice 30 d after irradiation with 7.2 Gy γ-rays. Blood and immunological experiments of mice were carried out to observe WBC (White blood cell), femoral nucleated cells, bone marrow DNA content and organs index after irradiation with 7.2 Gy γ-rays. Intestine pathological section of mice irradiated on abdomen with 9.0 Gy γ-rays was prepared to observe the pathology of intestine. The results showed that compared with the irradiation group, the survival rate of mice treated with PHA-L increased, especially for the high dose group (60% lifted); compared with the irradiation group, white blood cell count, bone marrow DNA content and spleen nodules of mice administered with high dose group increased from 0.74±0.16, 1.02±0.17 and 9.80±6.46 to 1.18±0.40, 1.22±0.17 and 18.10±6.87 respectively, which were statistically significant (p<0.05). Moreover, PHA-L also had some protective effect on the organ. In pathology observation, the intestines of mice treated with PHA-L recovered significantly as well. Therefore, plant lectin PHA-L can protect mice from radiation injuries, yet the mechanism needs further study.

Key words: Radiation damage, Plant lectin PHA-L, Radiation intestine

CLC Number: 

  • R979.6