Journal of Radiation Research and Radiation Proces ›› 2016, Vol. 34 ›› Issue (2): 20201-020201.doi: 10.11889/j.1000-3436.2016.rrj.34.020201

• RADIOBIOLOGY AND RADIOMEDICINE •     Next Articles

Irradiation protection effects of magnesium sulfate on human umbilical vein endothelial cells

SUN Yang, TU Yu   

  1. School of Radiation Medicine and Protection, Medical School of Soochow University, Suzhou 215123, China
  • Received:2016-01-19 Revised:2016-03-11 Online:2016-04-20 Published:2016-04-18
  • Supported by:

    Supported by National Natural Science Foundation of China (K112830513)

Abstract:

The human umbilical vein endothelial cells (HUVECs) of logarithmic growth phase were divided into three groups for different treatment. And then they were detected with the cell cycle distribution by flow cytometry, rate of apoptosis by annexin V/PI double staining method, expression of Bcl-2 protein by Western blot and expression of Bcl-2 mRNA by RT-PCR. The results showed that magnesium sulfate could advance the G2/M phase block of HUVECs after X-ray irradiation for 24 h, and improve the sensitivity of HUVECs to X-rays after irradiation for 24 h, 48 h and 72 h, increasing apoptosis rate to a certain extent (t=4.24, 7.19, 3.20, p<0.05) and decreasing Bcl-2 mRNA expression (t=3.034, 6.182, 3.957, p<0.05)after irradiation for 24 h, 48 h and 72 h, as well as inhibit the anti-apoptotic protein Bcl-2 protein expression significantly after irradiation for 48 h and 72 h. Therefore, magnesium sulfate could improve the G2/M phase arrest, increase the apoptosis rate and reduce Bcl-2 expression of HUVECs irradiated with X-rays.

Key words: Magnesium sulfate, Human umbilical vein endothelial cells, X-rays, Cell apoptosis, Bcl-2

CLC Number: 

  • TL71