Journal of Radiation Research and Radiation Proces ›› 2016, Vol. 34 ›› Issue (1): 10402-010402.doi: 10.11889/j.1000-3436.2016.rrj.34.010402

• RADIATION TECHNOLOGY APPLICATION • Previous Articles     Next Articles

Breeding pleuromutilin protoplasts strains by carbon ion beam irradiation

DU Wenyue1,2  LU Xihong1  LI Xuehu1  ZHOU Xiang1  LIANG Jianping1,2  XIN Zhijun1   

  1. 1(Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China)
    2(Life Science and Engineering College, Lanzhou University of Technology, Lanzhou 730000, China)
  • Received:2015-09-10 Revised:2015-12-16 Online:2016-02-20 Published:2016-01-18
  • Contact: Ph.D. LI Xuehu, associate professor E-mail:lixh@impcas.ac.cn
  • About author:DU Wenyue, female, was born in June 1988 and graduated from Gansu Agricultural University. Now she is a master candidate of Lanzhou University of Technology, majoring in microbial mutation breeding
  • Supported by:

    Supported by National High Technology Research and Development Program of China (863Program)(2011AA10A214) and Gansu Local Pharmaceutical Industry Development Fund (Y361010SJ0)

Abstract:

Clitopilus pinsitus protoplast was irradiated by 12C6+ ion beam at varied absorbed doses for mutagenesis, and high-yielding pleuromutilin mutant strains were screened using high-throughput method and solid fermentation. The results showed that the optimum 12C6+ ion beam absorbed dose is 1.5 Gy, and under this condition the positive mutation rate is 37.58%. By agar column prescreening and 96-plate solid fermentation, the mutant strains, C.pin15I5E and C.pin15II6B, were selected. The yields of the strain increased by 16.17% and 15.47%, respectively, compared with that of the original strain. The research suggests that applying heavy ion irradiation to protoplast is a possible method to breed mutant strains.

Key words: Carbon ion beam, Irradiation, Clitopilus pinsitus protoplast, High-yielding mutant strain, Pleuromutilin

CLC Number: 

  • Q691.5,TL99