Journal of Radiation Research and Radiation Proces ›› 2016, Vol. 34 ›› Issue (1): 10401-010401.doi: 10.11889/j.1000-3436.2016.rrj.34.010401

• RADIATION TECHNOLOGY APPLICATION • Previous Articles     Next Articles

Biological effects of argon ion beams on dry seeds of Arabidopsis thaliana

FENG Hui1,2  LUO Shanwei1,2  DU Yan1  YU Lixia1,2  MU Jinhu1,2  CHEN Yuze1,3  LI Wenjian1  ZHOU Libin1   

  1. 1(Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China)
    2(University of Chinese Academy of Sciences, Beijing 100049, China)
    3(Gansu Agricultural University, Lanzhou 730070, China)
  • Received:2015-09-15 Revised:2015-11-18 Online:2016-02-20 Published:2015-12-07
  • Contact: Ph.D. LI Wenjian, professor;Ph.D. ZHOU Libin, associate professor;
  • About author:FENG Hui, female, was born in February 1992 and graduated from Northwest Normal University in June 2014. Now she is a master candidate of Institute of Modern Physics, Chinese Academy of Sciences, majoring in bioengineering and focusing on basic study of mutation breeding. E-mail:
  • Supported by:

    Supported by the National Natural Science Foundation of China (11205218, 11275171, 11405234), CAS "Light of West China" Program (29Y506020) and Youth Innovation Promotion Association of CAS(29Y506030)


To study the biological effects of argon ion beams provided by Heavy Ion Medical Accelerator in Chiba (HIMAC, Program No.13J404) in National Institute of Radiological Sciences (NIRS) on Arabidopsis thaliana, the postembryonic development of irradiated dry seeds, such as survival rate, root length, hypocotyls length, plant height and bolting rate, were investigated. The results showed that survival rate, root length, hypocotyls length, plant height and bolting rate decreased with the increasing doses. The variations of genomic DNA fingerprints of the irradiated samples were analyzed by using random amplified polymorphic DNA (RAPD) technique. RAPD results demonstrated that the genetic similarity coefficient between the controls and treatment groups were 0.556−0.944. But the similarity coefficients among treatment groups irradiated with different dosages had no obvious correlations. All the results indicate that M1 seedlings of Arabidopsis thaliana irradiated by argon ions has obvious biological effects on both the plant developments and genomic DNA variations.

Key words: Argon ion beams, Irradiation, Arabidopsis thaliana, Biological effects, Random amplified polymorphic DNA (RAPD)

CLC Number: 

  • Q691.5,TL99