Journal of Radiation Research and Radiation Proces ›› 2016, Vol. 34 ›› Issue (1): 10203-010203.doi: 10.11889/j.1000-3436.2016.rrj.34.010203

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Establishment of stable knock-down cell lines and function study of lnc-RI,a radiation-induced gene

CHANG Cheng1,2 SHEN Liping2 ZHANG Xueqing2 LI Lin2 LI Xiaoxin2 
WANG Zhidong2 ZHOU Pingkun1,2   

  1.  1(School of Public Health, University of South China, Hengyang 421000, China)
    2(Institute of Radiation and Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China)
  • Received:2015-09-17 Revised:2015-11-12 Online:2016-02-20 Published:2015-12-07
  • Contact: ZHOU Pingkun, Doctoral tutor, professor; Ph.D. WANG Zhidong, associate professor E-mail:zhoupk@bmi.ac.cn;wangzhidong1977@126.com
  • About author:CHANG Cheng, female, was born in June 1987 and graduated from North China University of Science and Technology in 2012. Now she is a master candidate in University of South China, majoring in public health and preventive medicine and focusing on health toxicology,E-mail:1109308002@qq.com
  • Supported by:

    Supported by the National Natural Science Foundation of China (No.81573083 and No.81402631)

Abstract:

To prepare the lentivirus granules interfering lnc-RI and establish HeLa cell lines consistently down-regulating lnc-RI, as well as discover its effects on radiation damage, the lentivirus granules bearing various interfering sequences were prepared by lnc-RI carrying specific interfering sequences in to interfering plasmid pGLV2. The estable cell lines were established after infecting HeLa cells with lentivirus granules. RT-PCR was employed to determine the inhibitory effects of various interfering sequences on lnc-RI expression. DNA damage of the established estable cell lines were induced by ionizing radiation sensitivity of the established cell lines which were determined by colony formation assay. The results showed that the lentivirus granules bearing various interfering sequences were successfully prepared. The estable HeLa cell lines infected by lentivirus were obtained and lnc-RI expression levels was down-regulated. The results of colony formation assay showed that radiation sensitivity of the estable cell lines were increased when treated with each dose gamma ray irradiation. Thus, it is preliminarily proved the radiation sensitivity of the established cell lines were increased when treated with each dose gamma ray irradiation.

CLC Number: 

  • TL71