Journal of Radiation Research and Radiation Proces ›› 2015, Vol. 33 ›› Issue (5): 50205-050205.doi: 10.11889/j.1000-3436.2015.rrj.33.050205

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Study on RPS27L gene as a marker of radiation damage

LI Xiaoxin1,2  HU Jiangwei3  ZHANG Xueqing2  SHEN Liping2  LI Lin2  CHANG Cheng2  WANG Zhidong2  CHEN Ying1,2   

  1. 1(Medical University of Anhui, Hefei 230032, China)
    2(Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China)
    3(The 307th Hospital of PLA, Beijing 100071, China)
  • Received:2015-06-10 Revised:2015-07-19 Online:2015-10-20 Published:2015-10-12

Abstract:

In order to explore RPS27L’s possibility as a biomarker of radiation damage, we studied its time-response and dose-response after irradiation as well as its background level. Three healthy adults’ peripheral blood was exposed to gamma-rays with 0, 0.5, 1, 2, 4, 6, and 10 Gy, separately and cultured for 2, 4, 8, 12, and 24 h respectively. RPS27L’s expression change was detected by quantitative real-time PCR. We also analyzed its background level in 37 individuals. The results showed that RPS27L had a significant difference at 4, 8, 12, 24 h and a great dose-independent response. There was also a uniform background level of RPS27L among different age and gender. Thus, RPS27L is of great potential to be a new biomarker of radiation.

Key words: Nuclear radiation, RPS27L, Gene expression change, Quantitative real-time PCR, Biomarker

CLC Number: 

  • TL71