Journal of Radiation Research and Radiation Proces ›› 2015, Vol. 33 ›› Issue (4): 40201-040201.doi: 10.11889/j.1000-3436.2015.rrj.33.040201

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Radioprotective effects of ginger oleoresin through Nrf2 pathway

LI Qing  XU Chang  WANG Yan  DU Liqing  WANG Qin  WANG Lu  LIU Qiang   

  1. (Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College,
    Tianjin 300192, China)
  • Received:2015-05-25 Revised:2015-06-17 Online:2015-08-20 Published:2015-07-08
  • Contact: Qiang LIU E-mail:liuqiang@irm-cams.ac.cn

Abstract:

The cell toxicity of different concentration of ginger oleoresin on mesenchymal stem cells (MSCs) was judged by MTT(Methyl thiazolyl tetrazolium). Flowing cytometry experiment was taken to observe the change of reactive oxygen species (ROS). Comet assay was used to detect the DNA damage. RT-PCR and Western Blot were used to detect the expression level of nuclear factor-E2-related factor 2 (Nrf2) protein and two typically recognized Nrf2 downstream genes, heme oxygenase-1 (HO-1) and NAD (P) H quinine oxidoreductase 1 (NQO1). The results showed that ginger oleoresin can reduce the level of ROS and the DNA damage of MSCs, induced by irradiation, and increase the expression of HO1 and NQO1 in mRNA and protein levels, which suggests that ginger oleoresin can reduce the oxidative damage of radiation, performing the role of radioprotective. Its mechanism may be related to Nrf2.

Key words: Ginger oleoresin, Mesenchymal stem cells (MSCs), Nuclear factor-E2-related factor 2 (Nrf2), Radioprotective

CLC Number: 

  • TL71