Journal of Radiation Research and Radiation Proces ›› 2015, Vol. 33 ›› Issue (3): 30101-030101.doi: 10.11889/j.1000-3436.2015.rrj.33.030101

• REVIEW •     Next Articles

Application of synchrotron radiation vacuum ultraviolet circular dichroism spectroscopy in the DNA structure research

ZHOU Peipei1,2  WANG Lihua2  LIU Huajie2  FAN Chunhai1,2   

  1. 1(Wenzhou Medical University, Wenzhou 325035, China)
    2(Jiading campus, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China)
  • Received:2015-03-24 Revised:2015-03-30 Online:2015-06-20 Published:2015-06-12

Abstract:

Circular dichroism (CD) spectroscopy is one of the powerful tools for DNA structure research. Currently, the general CD spectrometer can only obtain the CD signal with wavelength above 190 nm, and the noise is very high with the wavelength range of 190−220 nm because of the influences from the air, the solvents, and the salt ion concentration of the buffer, etc., making it have not much practical value. The development of the synchrotron radiation circular dichroism (SRCD) makes it possible to obtain the CD signal of the DNA structure in the region of the vacuum ultraviolet (VUV). And now it becomes a new powerful tool for studying the DNA structure. This work firstly introduces the principle and features of SRCD technique, especially its advantages in the DNA structure research. Then the DNA structures and the factors affecting the CD signal are also reviewed. Finally, some typical DNA structures are taken as samples to analyze their SRCD spectrum.

Key words: Circular dichroism, DNA structure, Synchrotron radiation, Vacuum ultraviolet

CLC Number: 

  • TL929