Journal of Radiation Research and Radiation Proces ›› 2014, Vol. 32 ›› Issue (6): 4-060203.doi: 10.11889/j.1000-3436.2014.rrj.32.060203

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Study on radiosensitization effect and mechanism of resveratrol on lung A549 cells

XING Xiaomeng  WANG Yan  DU Liqing  XU Chang  LIN Kaili  FAN Saijun  LIU Qiang   

  1. (Institute of Radiation Medicine, Chinese of Academy of Medical Sciences, Peking Union Medical College,
    Tianjin 300192, China)
  • Received:2014-09-03 Revised:2014-10-30 Online:2014-12-20 Published:2014-11-17
  • Contact: Qiang LIU E-mail:liuqiang@irm-cams.ac.cn

Abstract:

 We aim to investigate the effect and mechanism of resveratrol on radiosensitivity of lung cancer A549 cells. MTT was used to detect the cell toxicity of different concentrations of resveratrol on lung cancer A549 cells; clonogenic assay was performed to determine the function of growth inhibition. The DNA damage was detected by the comet assay. Western blot was taken to detect the expression level of Survivin. The results showed that the inhibitory effect of resveratrol on A549 cells increased with resveratrol concentration and time; the combined effect of resveratrol and radiation significantly increased the DNA damage of A549 cells induced by radiation(p<0.05), and reduced cloning efficiency as well as the expression level of Survivin. It can be concluded that resveratrol can enhance the radiation sensitivity of A549 cells, and maybe the radiosensitizing effect is achieved by suppressing the expression of Survivin protein.

Key words: Resveratrol, Lung cancer A549 cells, Radiosensitization, Survivin protein

CLC Number: 

  • TL71