Relationship between genetic single-nucleotide polymorphisms of LIG4 and HSPB1 and the ratio of tumor-decreased-size by radiotherapy in non-small cell lung cancer

LIU Dan1  GUO Genyan1  ZHAO Yuxia1  BAI Lu2

1. 1(Department of Radiation Oncology, the Fourth Affiliated Hospital of China Medical University, Shenyang 110032, China)
2(Department of Radiation Oncology, the First Affiliated Hospital of China Medical University, Shenyang 110001, China)
• Received:2014-06-23 Revised:2014-09-17 Online:2014-12-20 Published:2014-10-28

Abstract:

137 pathologically proved non-small cell lung cell patients were treated with thoracic three-dimensional conformal radiotherapy from the First Affiliated Hospital of China Medical University district between December 2009 and December 2011. The tumor was measured in pre-radiotherapy and radiotherapy 20 times to estimate the ratio of tumor decreased size. DNA was extracted by phenol-chloroform method from blood samples. Genetic single-nucleotide polymorphisms were divided by Taqman real-time PCR. All statistical analyses were performed with SPSS13.0. p<0.05 was confirmed statistically significant. T-test and analysis of variance were used to compare the data. Among the 137 patients, the average age was 60.1±5.33; the average ratio of tumor decreased size was 0.3474±0.191; squamous carcinoma were 87 cases; adenocarcinoma were 50 cases; male were 102 cases, while female were 35 cases;Ⅰstage were 9 cases,Ⅱstage were 26 cases, Ⅲ stage were 70 cases, and Ⅳstage were 32 cases; Smoking patient were 95 cases. The ratio of tumor-decreased-size of smoking patient was higher than that of no-smoking, respective 0.3755±0.1775 and 0.2840±0.2068, t=2.643, p=0.009. The ratio of tumor decreased size of LIG4rs1805388CC, CT and TT genotype were 0.3395±0.1802, 0.342±0.2106 and 0.5038±0.1892, respectively. The ratio of tumor-decreased-size of LIG4rs1805388TT genotype was higher than that of CC genotype through LSD method. There was no relationship between genetic single-nucleotide polymorphisms of HSPB1 and the ratio of tumor-decreased-size by radiotherapy in non-small cell lung cancer. The ratio of tumor-decreased-size by radiotherapy of smoking and LIG4rs180538TT genotype was higher than that of other patients.

CLC Number:

• R734.2