Journal of Radiation Research and Radiation Proces ›› 2014, Vol. 32 ›› Issue (4): 40101-040101.doi: 10.11889/j.1000-3436.2014.rrj.32.040101

• REVIEW •     Next Articles

Study on the imaging of subcellular organelles with superresolution microscopy

JIA Sisi1  LIANG Le1  DENG Suhui1,2  HOU Shangguo1  LIU Huajie1, 2  HUANG Qing1, 2  FAN Chunhai1, 2   

  1. 1(Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences,
     Jiading Campus, Shanghai 201800, China)
    2(Center of Bio-imaging, Shanghai Sychrotron Radiation Facility, Chinese Academy of Sciences,
     Jiading Campus, Shanghai 201800, China)
  • Received:2014-03-20 Revised:2014-04-24 Online:2014-08-20 Published:2014-06-06

Abstract:

This review focused on the developments of three common microscopes in cell biology including far-field light nanoscopy, soft X-ray microscopy based on the synchrotron radiation light source, and three-dimensional cryo-electron microscopy. Their mechanisms, applications in cell imaging and the existing problems are discussed. The correlative microscopy combining several imaging methods can supply more information and may provide a new insight into cell biology in the future.

Key words: Subcellular imaging, Fluorescence super-resolution microscopy, Soft X-ray microscopy, Three- dimensionalcryo-electronmicroscopy, Correlative microscopy

CLC Number: 

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