Journal of Radiation Research and Radiation Proces ›› 2012, Vol. 30 ›› Issue (6): 374-377.doi: 10.11889/j.1000-3436.2012.rrj.30.120611

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Effects of ionizing radiation on MC3T3 cells’proliferation and expression of Notch1 and Jagged1

TANG Quan1  YANG Bing1  ZHONG Leilei1  YANG Fujun1  ZHANG Xiaodong1  SHANG Jie2  WANG Qin1  HAN Ying1  FAN Tiqiang1  FAN Feiyue1  SUN Yuanming1   

  1. 1 (Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical
     Sciences and Peking Union Medical College, Tsinghua University, Tianjin 300192, China)
    2 (Department of Pharmacy, Tianjin Medical University General Hospital, Tianjin 300052, China)
  • Received:2012-06-19 Revised:2012-08-06 Online:2012-12-20 Published:2014-09-09

Abstract:

In order to investigate the effects of ionizing radiation on MC3T3 cells’ proliferation and expression changes of Notch1 and Jagged1 genes, MC3T3 cells were exposed to γ-rays with 0, 2 and 4 Gy, respectively. MTT assay and real-time PCR were adopted to detect the proliferation of MC3T3 cells and the changes of expression of Notch1 and Jagged1 genes which could be in association with proliferation and differentiation. When exposed to 2 and 4 Gy γ-rays irradiation, the MC3T3 cells' proliferation reduced (p<0.01, p<0.01). The expression of Notch1 decreased when treated with 2 Gy irradiation (p<0.05). The expression of Jagged1 regulated down when given 2 and 4 Gy irradiation (p<0.01, p<0.05). Irradiated MC3T3 cells’ proliferation potentialities reduced and Notch1 and Jagged1were regulated down.

Key words: Ionizing radiation, Osteoblast, MC3T3, Notch1, Jagged1

CLC Number: 

  • R336,R811.5