Journal of Radiation Research and Radiation Proces ›› 2012, Vol. 30 ›› Issue (5): 309-315.doi: 10.11889/j.1000-3436.2012.rrj.30.120510

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Study on radiation protection of WSC-DTPA nanoparticles

LIU Yunying1  ZHOU Xiaowang1  WAN Jianmei2  HU Danfei1  ZHANG Youjiu1   

  1. 1(School Of Radiation Medicine and Protection, Medical College of Soochow University, Jiangsu Province Key Laboratory of Radiation Medicine and Protection , Suzhou 215123, China)
    2(Experimental Center of Medical College, Soochow University, Suzhou 215123, China)
  • Received:2011-11-25 Revised:2012-01-15 Online:2012-10-20 Published:2014-09-11

Abstract:

To investigate the radiation protection of WSC-DTPA nanoparticles, N-acylation reaction and ion crossing-linking method were used to prepare nanoparticles in different remaining free amino groups. MTT assay was performed to evaluate their effects on the viability of the cells after γ-rays irradiation, while the capability of FITC-WSC-DTPA nanoparticles probe penetrating through cellular membrane was observed by Live Cell Station. The results show that WSC-DTPA polymers were successfully synthesized, in which the content free amino group is 92.7%, 74.3%, 1.59%, respectively. Compared with γ-rays alone group, it was shown that WSC, WSC nanoparticles and WSC-DTPA nanoparticles with the concentration more than 6.25 μg/mL and the free amino group content around 92.7% have good efficacy in radiation protection to BRL cells from damage induced by 6 Gy 60Co γ-rays by the higher survival rate, while WSC-DTPA nanoparticles with 1.59% free amino group content do not have radiation protection to BRL cells. The WSC nanoparticles and WSC-DTPA nanoparticles can penetrate into cellular within 2 h, while WSC-DTPA polymers could not pass through the membrane.

Key words: Water-soluble low-molecular weight chitosan(WSC), Diethylenetriamine pentaacetatic acid (DTPA), Nanoparticles, BRL cells, Radiation protection

CLC Number: 

  • R811.5,R93,Q2-33