Journal of Radiation Research and Radiation Proces ›› 2012, Vol. 30 ›› Issue (1): 53-57.doi: 10.11889/j.1000-3436.2012.rrj.30.120110

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Mutation breeding of Aspergillus niger for producing citric acid by heavy ion beam

HU Wei1,2  CHEN Jihong2  ZHANG Zhen1  LIU Jing2  WANG Shuyang2  LI Wenjian2   

  1. 1(College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730030, China)
    2(Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China)
  • Received:2011-06-27 Revised:2011-08-29 Online:2012-02-20 Published:2014-09-25

Abstract:

In order to screen high-producing-citric acid strains, the original strain 4# was irradiated with different doses of heavy ion beams 12C+6. The citric acid content in the fermentation broth was measured by acid-base neu-tralization. The results showed that the positive mutation rate reached the highest value of 21% as the heavy ion 12C+6 irradiation dose was 60 Gy, and the high producing citric acid strain H4002 was obtained after screening. Compared with the original strain, its fermentation content of citric acid was improved by 18%. The results suggest that high-producing-citric acid strain could be obtained after it was irradiated by heavy ion beams and the mutation effect was remarkable.

Key words: Aspergillus niger, Heavy ion beam, Mutation, Breeding

CLC Number: 

  • Q939.9,Q691.5,Q815