Journal of Radiation Research and Radiation Proces ›› 2012, Vol. 30 ›› Issue (1): 48-52.doi: 10.11889/j.1000-3436.2012.rrj.30.120109

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Semi-quantitative analysis of the five up-regulated genes in rice screened by genechip array underlying the implantation of the low-energy ion beams

FENG Yingrui1  CHEN Qiufang1  QIN Guangyong1  YA Huiyuan1,2  SHANG Supei1  GU Lingbiao1  NI Kuikui1   

  1. 1(Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou 450052, China)
    2(College of Life Sciences Luoyang, Normal University, Luoyang 471022, China)
  • Received:2011-07-13 Revised:2011-12-15 Online:2012-02-20 Published:2014-09-25

Abstract:

We select abnormal expression genes from the gene chips which detected the nitrogen ion beam irra-diated rice, and these genes (NBS-LRR, PGPS/D12, Pherophorin-S, aspartic endopeptidase) involve in stress response and cell number regulation. In order to investigate the characters of these genes expression in rice seedlings stage irradiated by the low-energy iron beam, mRNA were purified from seedlings at different growth stages ranging from 72 to 120 h after implantation (HAI) and analyzed by semi-quantitative RT-PCR. The results showed that different implantation doses could regulate expression level of NBS-LRR1, NBS-LRR2, Pherophorin-S precursor, Aspartic endopeptidase and PGPS/D12, providing the target genes for the molecular mechanism of ion beam mutation.

Key words: Ion beam irradiation, Rice, Gene expression, Semi-quantitative RT-PCR

CLC Number: 

  • Q691.8