Journal of Radiation Research and Radiation Proces ›› 2015, Vol. 33 ›› Issue (5): 50402-050402.doi: 10.11889/j.1000-3436. 2015.rrj.33.050402

• RADIATION TECHNOLOGY APPLICATION • Previous Articles     Next Articles

Effects on the sugar components and related enzyme activity of sucrose metabolism by carbon ion irradiation in sweet sorghum

YANG Taoliwei1,2  LIU Ruiyuan1,2  GU Wenting2  BI Yang1  LI Wenjian2  DONG Xicun2   

  1. 1 (Gansu Agricultural University, Lanzhou 730000, China)
    2(Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China)
  • Received:2015-06-24 Revised:2015-09-14 Online:2015-10-20 Published:2015-10-19

Abstract:

In order to reveal accumulation mechanism of different sugar components and effects on sucrose metabolism related enzyme activity by carbon ion irradiation, the dynamic changes of sucrose, fructose, glucose contents and sucrose metabolism related enzyme activity were studied in the sweet sorghum, which early-maturing mutants, KF1210-3 and KF1210-4 isolated from KFJT-CK irradiated by carbon ion beam, were used as experiment materials. The results indicated that the sucrose content of KF1210-3 and KF1210-4 were significantly increased at mature stage, the value being 147.58 mg/gFw and 135.46 mg/gFw, respectively. The activities of sucrose synthase synthesis (SSs), Sucrose-phosphate synthase(SPS) were increased significantly, while sucrose synthase cleavage (SSc), acid inverse (INVA), neutral inverse (INVN) were significantly decreased. Sucrose accumulation of the mutants resulted in synergistic action of SSs, SSc, SPS, INVN and INVA. Higher ability of the sucrose synthesis and lower sucrose hydrolysis behavior increased sucrose content of the two mutants. In brief, the promoting effect on sucrose content was induced by carbon ion irradiation in sweet sorghum, which provides theory evidence for mutation breeding and related research of sucrose metabolism.

Key words: Sweet sorghum, Carbon ion irradiation, Sucrose-metabolizing, Enzyme activity

CLC Number: 

  • S514,TL99